Regulation of K-Cl Cotransport: from Function to Genes
نویسندگان
چکیده
منابع مشابه
Characterization of glial cell K-Cl cotransport.
BACKGROUND The molecular mechanism of K-Cl cotransport (KCC) consists of at least 4 isoforms, KCC 1, 2, 3, and 4 which, in multiple combinations, exist in most cells, including erythrocytes and neuronal cells. METHODS We utilized reverse-transcriptase-polymerase chain reaction (RT-PCR) and ion flux studies to characterize KCC activity in an immortalized in vitro cell model for fibrous astrocy...
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Previous data indicate that adenosine 3',5'-cyclic monophosphate activates the epithelial basolateral Na+-K+-Cl-cotransporter in microfilament-dependent fashion in part by direct action but also in response to apical Cl- loss (due to cell shrinkage or decreased intracellular Cl-). To further address the actin dependence of Na+-K+-Cl-cotransport, human epithelial T84 monolayers were exposed to a...
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Neurotransmitter transporters are reported to mediate transmembrane ion movements that are poorly coupled to neurotransmitter transport and to exhibit complex "channel-like" behaviors that challenge the classical "alternating access" transport model. To test alternative models, and to develop an improved model for the Na+- and Cl--dependent gamma-aminobutyric acid (GABA) transporter, GAT1, we e...
متن کاملCoordinate modulation of Na-K-2Cl cotransport and K-Cl cotransport by cell volume and chloride.
Na-K-2Cl cotransporter (NKCC) and K-Cl cotransporter (KCC) play key roles in cell volume regulation and epithelial Cl(-) transport. Reductions in either cell volume or cytosolic Cl(-) concentration ([Cl(-)](i)) stimulate a corrective uptake of KCl and water via NKCC, whereas cell swelling triggers KCl loss via KCC. The dependence of these transporters on volume and [Cl(-)](i) was evaluated in m...
متن کاملFlow-induced expression of endothelial Na-K-Cl cotransport: dependence on K(+) and Cl(-) channels.
Steady laminar shear stress has been shown previously to markedly increase Na-K-Cl cotransporter mRNA and protein in human umbilical vein endothelial cells and also to rapidly increase endothelial K(+) and Cl(-) channel conductances. The present study was done to evaluate the effects of shear stress on Na-K-Cl cotransporter activity and protein expression in bovine aortic endothelial cells (BAE...
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ژورنال
عنوان ژورنال: Journal of Membrane Biology
سال: 2006
ISSN: 0022-2631,1432-1424
DOI: 10.1007/s00232-006-1002-5